Description in short: hl60
library_ID: mm
tissue: myeloblastic leukemia cel line HL60
origin: culture DMEM 10% FCS >> harvest at semi confluent
prep date: 12/10/91
prep by: K.Okubo
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: cytoplasmic RNA prepared by NP40 lysis of cytoplasmic membrane
RNA yield: 100 micrograms of total cytoplasmic RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 12/29/91
library size: >10^6 independent
library host: DH5
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Description in short: hl60/DMSO
library_ID: pm
tissue: myeloblastic cell line HL60 induced to differentiate
origin: HL60 cells treated with DMSO > culture for 72 hrs
prep date: 2/8/92
prep by: K.Okubo
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 2/10/92
library size: >10^6 independent
library host: DH5
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Description in short: hl60/TPA
library_ID: mp
tissue: myeloblastic cell line HL60 induced to differentiate
origin: HL60 cells treated with TPA > culture for 48hrs
prep date: 2/8/92
prep by: K.Okubo
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 2/10/92
library size: >10^6 independent
library host: DH5
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Description in short: Granulocyte
library_ID: gr
tissue: pooled bood from red cross
origin: ficol cfg
prep date:
prep by:
Institution: Hiroshima Univ.
contact: Hiroyasu Uchiyama
Comment: 0824-24-6561
RNA prep: Fast track
RNA yield:
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date:
library size: >10^6 independent
library host: DH5
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Description in short: CD4 T cell
library_ID: fr
tissue: CD4+Tcells
origin: 1x10^7 cells from 50ml blood healthy male (K.O.)selection with Dynabeads for CD4+
prep date: 3/16/95
prep by: K.Okubo
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: yeold=
RNA prep: Poly A+ batch prep with Quick Prep Micro/with
RNA yield: 1 microgram polyA RNA
Vector primer: pUC19 PstI tailed/ 1 microgram
cDNA synthesis: polyA+VP
construction date: 3/18/95
library size: >10^6 independent
library host: DH5
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Description in short: CD 8 T cell
library_ID: et
tissue: CD8+Tcells
origin: 1x10^7 cells from 50ml blood healthy male (K.O.)selection with Dynabeads for CD4+
prep date: 3/16/95
prep by: K.Okubo
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: yeold=
RNA prep: Poly A+ batch prep with Quick Prep Micro/with
RNA yield: 1 microgram polyA RNA
Vector primer: pUC19 PstI tailed/ 1 microgram
cDNA synthesis: polyA+VP
construction date: 3/18/95
library size: >10^6 independent
library host: DH5
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Description in short: tumor infiltrates
library_ID: ti
tissue: Tumor Infiltrating Lymphocytes
origin: human colon cancer enzymatical digetion and discontinuous Ficoll-Hypaque gradient
prep date: 10/19/94
prep by: Yasuhide Miyamoto
Institution: "1st department of medicine, Osaka Univ. Med. Sch."
contact: 06-879-3636
Comment: Cellular composition was confirmed by light microscopeas >90% lymphocytes
RNA prep: Total RNA prepared by AGPC
RNA yield: 50 micro grams/enough for a synthesis
Vector primer: pUC19-Pst Tailed/0.5 micrograms
cDNA synthesis: Total RNA as template/cut out from agarose after electrophoresis
construction date: " Oct., 1994"
library size: >10^6 independent
library host: DH5
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Description in short: retina
library_ID: re
tissue: neural_retina
origin: " Autopsy ,single eye "
prep date: 1993
prep by: Akiyo Shimizu
Institution: Kyoto Prefectural School of Medicine.
contact: 617-726-5604
Comment:
RNA prep:
RNA yield: a few microgram of polyA RNA
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: 2/13/92
library size: >10^6 independent
library host: DH5
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Description in short: cortex (parietal)
library_ID: cc
tissue: cerebral_cortex
origin: gyrus_precentralis
prep date: 3/24/94
prep by: Tamotsu Hashimoto
Institution: Kyoto Prefectural_U of Medicine
contact:
Comment:
RNA prep: GnCs_Ulcfg_oligodT
RNA yield: 10ug_polyA
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: 7/19/94
library size: >10^6 independent
library host: DH5
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Description in short: cortex (temporal)
library_ID: nc
tissue: cerebral_cortex_temporal
origin: temporal_cortex_of agematched control for za
prep date: 1994
prep by: Jack Wands
Institution: MGH Cancer center.
contact: 617-726-5604
Comment:
RNA prep:
RNA yield: a few microgram of polyA RNA
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: 7/23/94
library size: >10^6 independent
library host: DH5
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Description in short: cortex (Altzheimer)
library_ID: za
tissue: temporal lobe of Alzheimer's disease patient
origin: Autopsy` pt#10
prep date: 1994
prep by: Jack Wands
Institution: MGH Cancer center.
contact: 617-726-5604
Comment:
RNA prep: Gnd>Cs>oligodT
RNA yield: a few microgram of polyA RNA
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: 7/23/94
library size: >10^6 independent
library host: DH5
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Description in short: cerebellum
library_ID: ce
tissue: cerebellar cortex
origin: dissected from autopsy brain 5hrs after death
prep date: 3/21/93
prep by: Kohichi Itoh
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 2 micrograms of polyA RNA
Vector primer: pSp72 PstI 35T
cDNA synthesis: polyA+VP
construction date: 10/1/93
library size: >10^6 independent
library host: DH5
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Description in short: hippocampus
library_ID: hi
tissue: hippocampus
origin: dissected from autopsy brain 5hrs after death
prep date: 3/19/93
prep by: Kohichi Itoh
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl
RNA yield: 20 micrograms of total RNA
Vector primer: pUC119 PstI 35T
cDNA synthesis: total RNA +VP (Kohichi Itoh)
construction date: 10/1/93
library size: >10^6 independent
library host: DH5
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Description in short: caudate n.
library_ID: ca
tissue: caudate_nucleus
origin: resected from autopsy brain 5hrs after death
prep date: 3/19/93
prep by: Kohichi Itoh
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl
RNA yield: 20 micrograms of total RNA
Vector primer: pUC119 PstI 35T
cDNA synthesis: total RNA +VP (Kohichi Itoh)
construction date: 7/19/94
library size: >10^6 independent
library host: DH5
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Description in short: thalamus
library_ID: th
tissue: dissected from autopsy brain 5hrs after death
origin: thalamus
prep date: 3/19/93
prep by: Kohichi Itoh
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl
RNA yield: 20 micrograms of total RNA
Vector primer: pUC119 PstI 35T
cDNA synthesis: total RNA +VP (Kohichi Itoh)
construction date: 7/19/94
library size: >10^6 independent
library host: DH5
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Description in short: putamen
library_ID: pu
tissue: putamen
origin: dissected from autopsy brain 5hrs after death
prep date: 3/19/93
prep by: Kohichi Itoh
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl
RNA yield: 20 micrograms of total RNA
Vector primer: pUC119 PstI 35T
cDNA synthesis: total RNA +VP (Kohichi Itoh)
construction date: 7/19/94
library size: >10^6 independent
library host: DH5
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Description in short: corpus callosum
library_ID: cl
tissue: corpus_callosum
origin: "16-75mixed age and sex,sudden_death"
prep date:
prep by: "Lot48600,"
Institution: Clontech
contact:
Comment:
RNA prep: AGPC+oligodT
RNA yield:
Vector primer: pUC119
cDNA synthesis: polyA+VP
construction date:
library size: >10^6 independent
library host: XL2Blue
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Description in short: substantia nigra
library_ID: ni
tissue: substancia_nigra
origin: "16-75mixed age and sex,sudden_death"
prep date: Lot41772
prep by: Clontech
Institution: CLONTECH
contact:
Comment:
RNA prep: AGPC+oligodT
RNA yield:
Vector primer: pUC119
cDNA synthesis: polyA+VP
construction date:
library size: >10^6 independent
library host: XL2Blue
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Description in short: fetal brain
library_ID: bf
tissue: hman_fetal_brain_whole
origin: "whole_brain,16-32wks,m/fmix,spontaneous_abortion"
prep date:
prep by: LOT6120298
Institution: CLONTECH
contact:
Comment:
RNA prep: AGPC+oligodT
RNA yield:
Vector primer: pUC119
cDNA synthesis: polyA+VP
construction date: 4/7/98
library size: >10^6 independent
library host: XL2Blue
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Description in short: fetal neuron
library_ID: ne
tissue: fetal_neuron_primary
origin: mixed culture of trypsinized human fetus brain tissue (5/95)
prep date:
prep by: Suen U. Kim
Institution: British Columbia
contact: sukim@unixg.ubc.ca
Comment: networking cells above confluent astrocyte layer harvested after detatched from astrocytes by shaking
RNA prep: Lysed with TRIZOL 2ml/ppt with glycogen (Kim)
RNA yield: 5 micro grams/enough for a synthesis
Vector primer: pUC19-Pst Tailed/0.5 micrograms
cDNA synthesis: totalRNA+VP/cut out from agarose after electrophoresis
construction date: 8/15/95
library size: 300 transformants from 1/50 of 2nd strand
library host: DH5
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Description in short: astrocyte
library_ID: as
tissue: astrocytes_cultured
origin: human fetus brain
prep date: mixed culture of trypsinized brain tissue (5/95)
prep by: Suen U. Kim
Institution: British Columbia
contact: sukim@unixg.ubc.ca
Comment: growing cells bneath network of neuron cells/ harvested after removal of neuron by shaking
RNA prep: Lysed with TRIZOL 2ml/ppt with glycogen (Kim)
RNA yield: 5 micro grams total RNA/enogh for a synthesis
Vector primer: pUC19-Pst Tailed/0.5 micrograms
cDNA synthesis: VP+totalRNA/cut out from agarose
construction date: 8/15/95
library size: 300 transformants from 1/50 of 2nd strand
library host: DH5
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Description in short: Schwann cell
library_ID: sc
tissue: schwann cells_cultured
origin: mixed culture of fetal dorsal root ganglia (5/95)
prep date:
prep by: Suen U. Kim
Institution: British Columbia
contact: sukim@unixg.ubc.ca
Comment: "Out growth cell from DRG of fetus, fibroblastic morphology"
RNA prep: Lysed with TRIZOL 500ul/ppt with glycogen (Kim)
RNA yield: 20 micro grams/enough for two synthesis
Vector primer: pGEM3-Pst Tailed/0.5 micrograms
cDNA synthesis: VP+totalRNA/half stored as 1st synthesis/ cut out from agarose after electrophoresis
construction date: 8/15/95
library size: 200 transformants from 1/50 of 2nd strand
library host: DH5
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Description in short: neuroblast
library_ID: nb
tissue: neuroblastoma CHP134
origin: estableshed from
prep date: ##/93
prep by: MasaHiro Yokoyama
Institution: Japan Tobacco co. at I.M.C.B osaka-u.
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: total RNA prepared by guanidiumHCl/CsCl
RNA yield: 2-3 microgram of polyA RNA
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: ##/93
library size: >10^6 independent
library host: DH5
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Description in short: pituitary
library_ID: pi
tissue: whole pituitary gland led
origin: 16-70 yrs male/female 21 specimens pool
prep date: purchased 6/94
prep by:
Institution: CLONTECH
contact: Lot35278
Comment:
RNA prep:
RNA yield:
Vector primer: pUC19 Pst 40T 1.5ug
cDNA synthesis: polyA+VP
construction date: 6/26/94
library size: >10^6
library host: DH5
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Description in short: fat (subcutaneous)
library_ID: ap
tissue: subcutaneous adipose
origin: dissected after surgery normal weight female
prep date: 8/12/92
prep by: Iichiro Shimomura
Institution: OsakaU med school 2nd dep int.med.
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl >> CsTFA >> oligodT column
RNA yield: 10 microgram polyA
Vector primer: pUC19 PstI 22T
cDNA synthesis: polyA+VP
construction date: 8/12/92
library size: >10^6 independent
library host: DH5
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Description in short: intima aorta
library_ID: ae
tissue: primary cultured pooled aortic endothelium
origin: 4x10^7 cells
prep date: 8/12/92
prep by:
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: Gift from 4th dept intl. med. osaka
RNA prep: Fast Track I
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 22T
cDNA synthesis: polyA+VP
construction date: 8/19/92
library size: >10^6 independent
library host: DH5
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Description in short: osteoblast
library_ID: ob
tissue: primary cultured osteoblast
origin: Surgically resected bone from osteoarthritis pt(63F) >> treated with collagenase
prep date: 10/#/92
prep by: Jun Hashimoto
Institution: Dept. of Orthopeadics Osaka Univ.
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: Total RNA prepared by AGPC
RNA yield: 5 microgram of polyA RNA
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: 1/23/92
library size: >10^6 independent
library host: DH5
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Description in short: fibroblast
library_ID: fc
tissue: primary cultured fibroblast
origin: leg skin resected at surgery (F)
prep date: 4/#/93
prep by: Jun Hashimoto
Institution: Dept. of Orthopeadics Osaka Univ.
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: polyA RNA prepared by micro prep (Pharmacia)
RNA yield:
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date:
library size: >10^6 independent
library host: DH5
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Description in short: fat (mesentric)
library_ID: vf
tissue: visceral-fat
origin: " mestenteric fat of 38yrs old patient. normal weight, no metabolic disorders."
prep date: 1/15/93
prep by: Iichiro Shimomura
Institution: 2nd Dept. of Internal Med. Osaka Univ.
contact: kousaku@imcb.osaka-u.ac.jp
Comment: With proper informed consent and approved by Human Investigative Committee.
RNA prep: polyA RNA prepared by GuanidiumHCl-CsCl method.
RNA yield: 0.5 microgram of polyA RNA
Vector primer: PUC19-PstI
cDNA synthesis: polyA+VP
construction date: ##/93
library size: >10^6 independent
library host: DH5
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Description in short: mesangial cell
library_ID: an
tissue: primary cultured mesangium cell
origin: 7th passage
prep date: 5/#/94
prep by: Toshio Miyata
Institution: Dept. of Med. Nagoya Univ.
contact: 052-723-1111 ex580
Comment: "FavtorVIII-,VLA2-,3+,4-,5+"
RNA prep: CsTFA>>OligodT
RNA yield: 10 micrograms polyA RNA
Vector primer: pUC19PstI 30T(1ug)
cDNA synthesis: polyA+VP
construction date: 6/19/94
library size: 10^6
library host: DH5
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Description in short: Itoh cell
library_ID: it
tissue: cultured_human itoh cells
origin: " 3 to 4x10^6 human myofibroblastic itoh cells(line40, passage2, male)"
prep date: 7/28/94
prep by: Jean Rosenbaum
Institution: Unite de Recherches INSERM U-99
contact: 33-3-49-81-35-30
Comment:
RNA prep: Lysed with TRIZOL2ml/ppt with glycogen (Dr.Rosenbaum)
RNA yield: ployA selected by microprep kitpolyA yeild about 1 microgram
Vector primer: pUC119PstI (0.5 microgram)
cDNA synthesis: polyA+VP
construction date: 1/31/94
library size: 10^4
library host: DH5
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Description in short: reaming bone
library_ID: bx
tissue: acetablum cancellous bone reamed flakes
origin: pooled female specimen / total hip joint rp.
prep date: 1995/1/25
prep by: Jun Hashimoto
Institution: Dept. of Orthopeadics Osaka Univ.
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: total RNA prepared by guanidiumHCl/CsCl
RNA yield: 2-3 microgram of polyA RNA
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: 8/13/95
library size: >10^6 independent
library host: DH5
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Description in short: media aorta
library_ID: am
tissue: aortic_media
origin: peeling intima and externa off from thoracic and abdominal aorta
prep date: 7/31/95
prep by: Kentaro Shimokado
Institution: National Center for Cardiovascular Disease
contact: 06-833-5012
Comment: Death delivery due to hiatus herniation (7/31/95)
RNA prep: Lysed with 4x(1cm x 1cm x 2mm) in TRIZOL4ml/ppt with glycogen (Kawamoto)
RNA yield: 20 micrograms purified with microprep >> polyA yeild about 1ug
Vector primer: pUC19-PstI tailed
cDNA synthesis: polyA+VP
construction date: 8/15/95
library size: 50 transformants from 1/50 of 2nd strand
library host: DH10B (electro competent)
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Description in short: fat (mesentric) (2)
library_ID: vg
tissue: visceral-fat
origin: " mestenteric fat of 38yrs old patient. normal weight, no metabolic disorders."
prep date: 1/15/93
prep by: Iichiro Shimomura
Institution: 2nd Dept. of Internal Med. Osaka Univ.
contact: kousaku@imcb.osaka-u.ac.jp
Comment: With proper informed consent and approved by Human Investigative Committee.
RNA prep: polyA RNA prepared by GuanidiumHCl-CsCl method.
RNA yield: 0.5 microgram of polyA RNA
Vector primer: PUC19-PstI
cDNA synthesis: polyA+VP
construction date: ##/93
library size: >10^6 independent
library host: DH5
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Description in short: ventricle muscle
library_ID: vt
tissue: left ventricle
origin: autopsy 35yrs male c no histry of cardiac drug administration
prep date: 2/10/97
prep by: K.shimokado
Institution: Institute.for CardiovascularDiseases
contact: kshimoka@res.ncvc.go.jp
Comment:
RNA prep: AGPC
RNA yield: 100microgram of totalRNA
Vector primer: pUC19
cDNA synthesis: totalRNA +VP
construction date: 2/24/97
library size: 10^4
library host: DH12S EP
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Description in short: atrial muscle
library_ID: ra
tissue: atrial_muscle
origin: "autopsy 35yrs male c no histry of cardiac drug administration, right_auricle"
prep date: 2/10/97
prep by: K.shimokado
Institution: Institute.for CardiovascularDiseases
contact: kshimoka@res.ncvc.go.jp
Comment:
RNA prep: AGPC
RNA yield: 100microgram of totalRNA
Vector primer: pUC19
cDNA synthesis: totalRNA +VP
construction date: 2/24/97
library size: 10^4
library host: DH12S EP
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Description in short: skeletal muscle
library_ID: sk
tissue: human skeletal muscle
origin: "CLONTECH, mixed sex age "
prep date: Lot7050554
prep by:
Institution: CLONTECH
contact:
Comment:
RNA prep: guanidin
RNA yield: polyA 5ug
Vector primer: pUC19
cDNA synthesis: polyA+VP
construction date: 8/12/97
library size: >10^6 independent
library host: DH5
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Description in short: keratinocyte
library_ID: ke
tissue: primary cultured pooled keratinocyte (NHDF648) (Kurabo)
origin: 4x 200 ml flask
prep date: 10/20/92
prep by:
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: Gift from Kurabo
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 2/13/93
library size: >10^6 independent
library host: DH5
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Description in short: cornea
library_ID: co
tissue: corneal epithelium
origin: scraped from 18 normal eyes before laser surgery
prep date: 4/93-10/93
prep by: Kouji Nishida
Institution: "Dept. of Ophthalmology, Kyouto Prefectural-U of Med "
contact:
Comment: With proper informed consent and approved by Human Investigative Committee of Kyoto Pref. Uni
RNA prep: polyA RNA prepared by micro prep (Pharmacia)
RNA yield: 1 microgram of polyA RNA
Vector primer: Psp72
cDNA synthesis: polyA+VP
construction date: ##/93
library size: 10^4
library host: DH5
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Description in short: taste bud
library_ID: tb
tissue: surgically resected tong with ZK
origin: papillae enucleated from tung (0.1ml)
prep date: 2/19/94
prep by: Katsumi Doi
Institution: Dept. of ORP Osaka Univ.
contact: 06-879-3951
Comment:
RNA prep: Lysed with TRIZOL2ml/ppt with glycogen
RNA yield: 50 micrograms total RNA
Vector primer: pUC119PstI(0.25ug)
cDNA synthesis: polyA+VP
construction date: 2/19/94
library size: 10^4
library host: DH5
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Description in short: cornea(2)
library_ID: cr
tissue: corneal epithelium
origin: scraped from 18 normal eyes before laser surgery
prep date: 4/93-10/93
prep by: Kouji Nishida
Institution: "Dept. of Ophthalmology, Kyouto Prefectural-U of Med "
contact:
Comment: With proper informed consent and approved by Human Investigative Committee of Kyoto Pref. Uni
RNA prep: polyA RNA prepared by micro prep (Pharmacia)
RNA yield: 1 microgram of polyA RNA
Vector primer: Psp72
cDNA synthesis: polyA+VP
construction date: ##/93
library size: 10^4
library host: DH5
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Description in short: conjunctiva
library_ID: cj
tissue: conjunctival_epithelium
origin: smeared_health_volunteer
prep date:
prep by: NIshidaK
Institution: "Dept. of Ophthalmology, Kyouto Prefectural-U of Med "
contact:
Comment:
RNA prep: AGPC
RNA yield: 10mgfrom30eyes
Vector primer: pUC119
cDNA synthesis: VP+total
construction date:
library size: 10^4
library host: XL2B
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Description in short: fundic gland
library_ID:
tissue: fundic_gland_epithelial_prep(Nemmakun)_Middle_Lesser
origin: BrIII_pyrolusK
prep date: 10/14/97
prep by: Tadashi_ohnishi
Institution: "2nd dept surgery, Osaka-U"
contact:
Comment:
RNA prep: RNAEZ
RNA yield: 5microgram
Vector primer: 0.2ug pUC119
cDNA synthesis: VP+totalRNA
construction date: 10/25/97
library size: 10^5
library host: DH12S
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Description in short: intestinal metaplasia
library_ID:
tissue: intestinal_metaplasia_epithelial_prep(Nemmakun)_Middle_Lesser
origin: "age?male,distal Gx for IIc+III"
prep date: 8/14/97
prep by: Tadashi_ohnishi
Institution: "2nd dept surgery, Osaka-U"
contact:
Comment:
RNA prep: RNAEZ
RNA yield: 10microgram
Vector primer: 0.5ugpUC119
cDNA synthesis: totalRNA +VP
construction date: 8/14/98
library size: 10/4/97
library host: DH12S
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Description in short: ileum end
library_ID:
tissue: ileum_end_epithelial_prep(Nemmakun)
origin: "10cm oral from BauhinV, caecum ca. 63male"
prep date: 10/30/97
prep by: Tadashi_ohnishi
Institution: "2nd dept surgery, Osaka-U"
contact: "tohnishi@imcb.osaka-u,.ac.jp"
Comment: almost_no_contaminating_non_epithelial_cells_proved
RNA prep: RNAEZ
RNA yield: 5microgram
Vector primer: 0.2ug pUC119
cDNA synthesis: VP+totalRNA
construction date: 1/29/98
library size: 10^5
library host: DH12S
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Description in short: astrocyte
library_ID: as
tissue: ascending_colon_epithelium_Nemmakun
origin: normal mucosa_of ileal ca. pt
prep date: 8/31/98
prep by: OhnishiT
Institution: "2nd dept surgery, Osaka-U"
contact:
Comment: almost_no_contaminating_non_epithelial_cells_proved
RNA prep: RNAEZ
RNA yield: 17micrograms
Vector primer: 0.2ug pUC119
cDNA synthesis: total+VP
construction date: 11/16/98
library size: 10^5
library host: XLIIB
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Description in short: sigmoid colon
library_ID:
tissue: sigmoid_colon_epithelial_isolate_Nemmakun
origin: "53yrmale,RbKSm,LAR,30cmfromAV"
prep date: 1/8/98
prep by: Tadashi_ohnishi
Institution: "2nd dept surgery, Osaka-U"
contact: "tohnishi@imcb.osaka-u,.ac.jp"
Comment: almost_no_contaminating_non_epithelial_cells_proved
RNA prep: RNAEZ
RNA yield: 5microgram
Vector primer: 0.2ug pUC119
cDNA synthesis: VP+totalRNA
construction date: 1/14/98
library size: 10^5
library host: DH12S
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Description in short: sigmoid colon (2)
library_ID:
tissue: sigmoid_colon_epithelial_isolate_Nemmakun
origin: "53yrmale,RbKSm,LAR,30cmfromAV"
prep date: 1/8/98
prep by: Tadashi_ohnishi
Institution: "2nd dept surgery, Osaka-U"
contact: "tohnishi@imcb.osaka-u,.ac.jp"
Comment: almost_no_contaminating_non_epithelial_cells_proved
RNA prep: RNAEZ
RNA yield: 5microgram
Vector primer: 0.2ug pUC119
cDNA synthesis: VP+totalRNA
construction date: 1/14/98
library size: 10^5
library host: DH12S
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Description in short: hepatoblastoma
library_ID: a
tissue: hepG2 hepatoblastoma / cocasian boy
origin: culture DMEM 10%FCSharvest at semi confluent
prep date: 10/20/90
prep by: K.Okubo
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: cytoplasmic RNA prepared by NP40 lysis of cytoplasmic membrane >> oligodT cellurose
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 10/20/90
library size: >10^6
library host: DH5
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Description in short: neonatal liver
library_ID: fl
tissue: "fetal_liver, 19 weeks of gestation"
origin: fetal liver from aborted fetus at 19 weeks of gestation
prep date:
prep by:
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: Fast Track
RNA yield: 2-3 micrograms polyA+RNA
Vector primer: pUC19 PstI 22T
cDNA synthesis: polyA+VP
construction date: 6/18/92
library size: >10^6
library host: DH5
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Description in short: fetal liver
library_ID: lf
tissue: neonatal_liver
origin: liver from a boy died at deriverly
prep date: ?/?/89
prep by: Shiozawa Hideyuki
Institution: Keioh Univ
contact: kousaku@imcb.osaka-u.ac.jp
Comment: Stored for 4 years in -80C as mass
RNA prep: guanidium HCl >> CsTFA >> oligodT column
RNA yield: >10 micrograms of polyA RNA
Vector primer: pUC19 PstI 22T
cDNA synthesis: polyA+VP
construction date: 6/23/92
library size: >10^6
library host: DH5
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Description in short: adult liver
library_ID: al
tissue: adult_liver
origin: normal part of resected liver with metastatic coloncancer
prep date:
prep by:
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl >> CsTFA >> oligodT column
RNA yield: 5 micrograms of polyA
Vector primer: pUC19 PstI 22T
cDNA synthesis: polyA+VP
construction date: 6/23/92
library size: >10^6
library host: DH5
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Description in short: lung
library_ID: lg
tissue: normal part of resected lung bearing lung cancer (male)
origin: peripheral part of lung was dissected after surgery
prep date: 07/??/92
prep by: Hideoki Yokouchi
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 2-3 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 8/13/92
library size: >10^6
library host: DH5
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Description in short: colonic mucosa
library_ID: cm
tissue: sigmoid colon mucosa
origin: m+sm peeled off from surgically resected colon/male with colon ca. 77 yr
prep date: 2/29/92
prep by: "Kousaku Okubo, Hideoki Yokouchi"
Institution: IMCB
contact: kousaku@imcb.osaka-u.ac.jp
Comment:
RNA prep: lysed in guanidium HCl >> pellet in CsTFA
RNA yield: 2-3 micrograms of polyA RNA
Vector primer: puc19 PstI 22T
cDNA synthesis: polyA+VP
construction date: 6/12/92
library size: >10^6
library host: DH5
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Description in short: iris
library_ID:
tissue: iris_glaucoma
origin: resected from glaucoma_blind_Pt57M
prep date: 980223
prep by: Hirofumi Shibuya
Institution:
contact:
Comment:
RNA prep: AGPC
RNA yield:
Vector primer: pUC119
cDNA synthesis: VP+total
construction date: 980515
library size: 10^5
library host:
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Description in short: small cell carcinoma
library_ID: sm
tissue: surgically resected cancer mass of small cell carcinoma (male)
origin: ssamll_cell_carci_ling
prep date: ##/##/92
prep by: Kouichi Itoh
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: histology available
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 2-3 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 11/10/92
library size: >10^6
library host: DH5
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Description in short: adenocarcinoma
library_ID: ad
tissue: surgically resected cancer mass of
origin: adenocarci_lung
prep date: ##/##/92
prep by: Hideoki Yokouchi/Kohichi Itoh
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: histology available
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 11/10/92
library size: >10^6
library host: DH5
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Description in short: squamous cell carcinoma
library_ID: sq
tissue: surgically resected cancer mass of squamous cell carcinoma (male)
origin: squamous_cell_carci_lung
prep date: ##/##/92
prep by: Hideoki Yokouchi
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: histology available
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 35T
cDNA synthesis: polyA+VP
construction date: 11/13/92
library size: >10^6
library host: DH5
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Description in short:
library_ID: uv
tissue: primary cultured pooled umbilical vein
origin: 1x10^7 cells
prep date: 10/15/92
prep by:
Institution: "IMCB, Osaka_u"
contact: kousaku@imcb.osaka-u.ac.jp
Comment: Gift from Kurabo
RNA prep: guanidium HCl/CsCl >> OligodT cellurose
RNA yield: 5 micrograms of polyA RNA
Vector primer: pUC19 PstI 22T
cDNA synthesis: polyA+VP
construction date: 10/19/92
library size: not transformed yet
library host: DH5
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